3D Bioplotter Research Papers

Cerebral organoids (cORGs) obtained from induced pluripotent stem cells (iPSCs) have become significant instruments for investigating human neurophysiology, with the possibility of simulating diseases and enhancing drug discovery. The current approaches require a strict process of manual inclusion in animal-derived matrix Matrigel® and are challenged by unpredictability, operators’ skill and expertise, elevated costs, and restricted scalability, impeding their extensive applicability and translational potential. In this study, we present a novel method to generate brain organoids that address these limitations. Our approach does not require a manual, operator-dependent embedding. Instead, it employs a chemically defined hydrogel in which the Matrigel® is…

The fabrication of three-dimensional (3D) bioprinted free-standing, low viscous, cell-laden hydrogels with good resolution, low cytotoxicity, and mechanical properties, comparable to native soft tissues, is a current challenge in tissue engineering. Recently, a new syringe extrusion approach, called Freeform Reversible Embedding of Suspended Hydrogels (FRESH), has been introduced to enhance 3D-bioprinting of soft hydrogels. Printing is conducted with the material embedded in a thermo-reversible gelatin bath, which acts as supporting material and can also initiate in-situ crosslinking when proper crosslinker agents are added. This work is the first to develop a 3D FRESH printable, low-cost, polymeric hydrogel composed of sodium…

Bioprinting consists in an innovative approach able to improve the current techniques of bioregeneration in the medical field through the extrusion of cell-loaded bioinks. Its main advantage is the customization to reduce post-operative complications on the patient, as it can be produced from his own cells. The success of bioprinting is determined by the printing parameters but, above all, by the materials. The goal of this work was to define a range of parameters, in order to achieve the highest printing stability, in terms of the quality of the Bioplotter® Silicone TG in relation to process conditions used.

In recent years, a new additive manufacturing (AM) method for three-dimensional (3D) syringe-extrusion (bio)printing of soft hydrogels has been introduced under the name of Freeform Reversible Embedding of Suspended Hydrogels (FRESH). The most common FRESH bath contains gelatin as the main compound and low concentrations of crosslinker(s) (whose nature depends on the hydrogel) for the initiation of an in-situ pre-crosslinking process during printing. In the case of sodium alginate (SA)-based hydrogels ionically crosslinked via calcium chloride (CaCl2), the crosslinker percentage in the gelatin bath is equal to ~10 mM, usually combined with a post-crosslinking at higher concentrations. However, according to the…