3D Bioplotter Research Papers

Bioprinting consists in an innovative approach able to improve the current techniques of bioregeneration in the medical field through the extrusion of cell-loaded bioinks. Its main advantage is the customization to reduce post-operative complications on the patient, as it can be produced from his own cells. The success of bioprinting is determined by the printing parameters but, above all, by the materials. The goal of this work was to define a range of parameters, in order to achieve the highest printing stability, in terms of the quality of the Bioplotter® Silicone TG in relation to process conditions used.

In recent years, a new additive manufacturing (AM) method for three-dimensional (3D) syringe-extrusion (bio)printing of soft hydrogels has been introduced under the name of Freeform Reversible Embedding of Suspended Hydrogels (FRESH). The most common FRESH bath contains gelatin as the main compound and low concentrations of crosslinker(s) (whose nature depends on the hydrogel) for the initiation of an in-situ pre-crosslinking process during printing. In the case of sodium alginate (SA)-based hydrogels ionically crosslinked via calcium chloride (CaCl2), the crosslinker percentage in the gelatin bath is equal to ~10 mM, usually combined with a post-crosslinking at higher concentrations. However, according to the…