3D Bioplotter Research Papers

In pre-hospital care, achieving rapid and effective hemostasis for arterial rupture and visceral perforation wounds remains a critical challenge. Herein, we have developed a macroporous sponge with double-network structure using foaming technique, chemical and physical crosslinking reactions, and lyophilization. The prepared sponge not only demonstrates outstanding water absorption and water-triggered shape recovery capacity, but also exhibits significantly enhanced mechanical properties due to the construction of double-network structure. Simultaneously, the sponge shortens blood clotting time (from 1354.3 ± 41 s to 473.0 ± 28 s) by concentrating blood components and regulating coagulation pathways. Particularly, the sponge possesses excellent 3D printability and…

Mg-stabilised Na-β’’-alumina solid electrolyte (Mg-BASE) for Na-ion batteries was synthesised and fabricated into 3D structures via direct ink writing (DIW), an extrusion-based additive manufacturing process. To produce a water-based ink with optimum viscoelastic properties and supreme printing quality, a comprehensive investigation of ink formulation and printing parameters was conducted. The sintered 3D structures of Mg-BASE, fabricated via direct ink writing, achieved relative density of 98.0 ± 1.1 % with β’’ phase fraction of 99.7 wt% whilst bulk ionic conductivity of 0.081 S⋅cm−1 at 350 °C was obtained. XRD results indicated that Mg-BASE fabricated via DIW may have different c-axis orientation than conventional dry-pressed pellets, leading to…

Extrusion-based three-dimensional (3D) printing of gelatin (Gel) is crucial for fabricating bone tissue engineering scaffolds via additive manufacturing. However, the thermal instability of Gel remains a persistent challenge, as it tends to collapse at mild temperatures. Current approaches often involve simply mixing Gel particles with various materials, resulting in biomaterial inks that lack uniformity and have inconsistent degradation characteristics. In this study, acetic acid was used to dissolve Gel and polycaprolactone (PCL) separately, producing homogeneous Gel/PCL dispersions with optimal pre-treatment performance. These dispersions were then combined and hybridized with nano-hydroxyapatite (n-HA) to create a composite printing ink. By evaluating the…

The growth plate (GP) is a crucial tissue involved in skeleton development via endochondral ossification (EO). The bone organoid is a potential research model capable of simulating the physiological function, spatial structure, and intercellular communication of native GPs. However, mimicking the EO process remains a key challenge for bone organoid research. To simulate this orderly mineralization process, we designed an in vitro shCav3.3 ATDC5-loaded gelatin methacryloyl (GelMA) hydrogel model and evaluated its bioprintability for future organoid construction. In this paper, we report the first demonstration that the T-type voltage-dependent calcium channel (T-VDCC) subtype Cav3.3 is dominantly expressed in chondrocytes and…

Polycaprolactone (PCL), either in its pure grade or as a polymeric matrix for bio-composites, plays a key role in the biomedical and bioengineering industries. It is also considered a multifunctional and versatile polymer for bioprinting and bioplotting purposes, especially in tissue engineering. Herein, an undiscovered yet valuable aspect of PCL extrusion-based bioprinting, such as the predictability of Critical Quality Indicators (CQIs), is investigated in depth. With the aid of the robust L25 orthogonal matrix design, the six most generic and device-independent control factors proved their impact on quality metrics such as global porosity, dimensional conformity, and surface roughness, determined with…

Bioplotting has high potential for the 3D printing of scaffolds and cellular structures. Medical-grade poly(ε-caprolactone) (PCL) is characterized by low 3D printing temperatures and strengths compared to those of common polymers. Thus far, research on PCL in bioplotting has mainly focused on improving its performance through the development of composites. In this study, the quantitative impact of six common 3D plotting settings on the engineering strength of PCL parts was evaluated. Three modeling approaches were implemented: linear regression modeling (LRM), reduced quadratic regression modeling, and quadratic regression modeling. The LRM results were not as accurate as those obtained using the…

Melanoma is one of the most aggressive types of skin cancer, and the need for advanced platforms to study this disease and to develop new treatments is rising. 3D bioprinted tumor models are emerging as advanced tools to tackle these needs, with the design of adequate bioinks being a fundamental step to address this challenging process. Thus, this work explores the synergy between two biobased nanofibers, nanofibrillated cellulose (NFC) and lysozyme amyloid nanofibrils (LNFs), to create pectin nanocomposite hydrogel bioinks for the 3D bioprinting of A375 melanoma cell-laden living constructs. The incorporation of LNFs (5, 10 or 15 wt%) on a…

The integrated repair of cartilage and bone involves the migration and differentiation of cells, which has always been a difficult problem to be solved. We utilize the natural biomaterial gelatin to construct gelatin methacryloyl (GelMA), a hydrogel scaffold with high cell affinity. GelMA is mixed with different components to print a bi-layer porous hydrogel scaffold with different modulus and composition in upper and lower layers through three-dimensional (3D) printing technology. The upper scaffold adds black phosphorus (BP) and human umbilical cord mesenchymal stem cells (hUMSCs) exosomes (exos) in GelMA, which has a relatively lower elastic modulus and is conducive to…

Auxetic scaffolds fabricated via additive manufacturing can enable cyclic mechanical stimulation to promote the biomechanical functionalization of engineered tissues. Typical designs of additively manufactured scaffolds used in tissue engineering literature (e.g., 0/90˚ strand laydown) are not amenable to cyclic loading due to their rigidity, which is in part due to the high stiffness of biopolymers such as polycaprolactone (PCL). Auxetic scaffolds can help overcome this due to their design-induced elasticity while recapitulating negative Poisson’s ratios seen in various natural tissues. In this study, we investigated the effects of auxetic design patterns and unit cell sizes on the mechanical properties of…

The fabrication of three-dimensional (3D) bioprinted free-standing, low viscous, cell-laden hydrogels with good resolution, low cytotoxicity, and mechanical properties, comparable to native soft tissues, is a current challenge in tissue engineering. Recently, a new syringe extrusion approach, called Freeform Reversible Embedding of Suspended Hydrogels (FRESH), has been introduced to enhance 3D-bioprinting of soft hydrogels. Printing is conducted with the material embedded in a thermo-reversible gelatin bath, which acts as supporting material and can also initiate in-situ crosslinking when proper crosslinker agents are added. This work is the first to develop a 3D FRESH printable, low-cost, polymeric hydrogel composed of sodium…