3D Bioplotter Research Papers

Displaying all papers by R. Engel (1 results)

Three-dimensional cell culture approach for in vitro immunization and the production of monoclonal antibodies

Biomedical Materials 2022 Volume 17, Number 5, Article 055003

The generation of monoclonal antibodies using an in vitro immunization approach is a promising alternative to conventional hybridoma technology. As recently published, the in vitro approach enables an antigen-specific activation of B lymphocytes within 10–12 d followed by immortalization and subsequent selection of hybridomas. This in vitro process can be further improved by using a three-dimensional surrounding to stabilize the complex microenvironment required for a successful immune reaction. In this study, the suitability of Geltrex as a material for the generation of monoclonal antigen-specific antibodies by in vitro immunization was analyzed. We could show that dendritic cells, B cells, and…