Silicone resin derived larnite/C scaffolds via 3D printing for potential tumor therapy and bone regeneration

Chemical Engineering Journal 2020 Volume 382, Article 122928

Three dimensional (3D) printing has been used to fabricate bioceramic scaffolds for treating the tumor-related defects in recent years, but the fabrication process and the introduction of anti-tumor agents are still challenging. In this study, porous free carbon-embedding larnite (larnite/C) scaffolds have been successfully fabricated by 3D printing of the silicone resin loaded with CaCO3 filler and high temperature treatment under an inert atmosphere. The fabricated larnite/C scaffolds had uniform interconnected macropores (ca. 400 μm), and exhibited excellent photothermal effect, which was able to kill human osteosarcoma cells (MNNG/HOS) and inhibit the tumor growth in nude mice. Moreover, the larnite/C scaffolds could stimulate the expression of osteogenesis-related gene (ALP, OCN and Runx-2) in rat bone mesenchymal stem cells (rBMSCs), and also promoted new bone formation in critical-sized rat calvarial defects. Therefore, the combination of 3D printing with polymer-derived ceramics strategy could fabricate multifunctional bioceramic scaffolds, which would be promising for potential application in treating tumor-related bone defects.