Testing mesenchymal stem cells on biocompatible 3D scaffold
The composite, thermoplastic material composed of polyhydroxybutyrate (PHB) and polylactic acid (PLA) was seeded with stem cells in the experiment. Tests of the polymer were oriented towards biocompatibility in vitro using mesenchymal stem cells isolated from the chorion. PHB/PLA is a currently tested biopolymer for applications in and medicine. Using additive technology, 3D forms of scaffolds in the form of a grid were prepared, which were seeded with stem cells and cultivated in suitable conditions. After an interval of 5 days, the proliferation and viability of the mesenchymal stem cells was tested by the proliferation test. From the results, it was found that the PHB/PLA material was a suitable scaffold and 60-85% stem cell viability was observed. Testing of non-toxic and degradable biopolymers brings new solutions in therapy in clinical orthopedic practice.