3D Bioplotter Research Papers

Displaying all papers by M. Costantini (3 results)

Alginate-based tissue-specific bioinks for multi-material 3D-bioprinting of pancreatic islets and blood vessels: A step towards vascularized pancreas grafts

Bioprinting 2021 Volume 24, Article e00163

Although allogeneic islet transplantation has been proposed as a therapy for type 1 diabetes, its success rate remains low. Disruption of both extracellular matrix (ECM) and dense vascular network during islets isolation are referred to as some of the main causes of their poor engraftment. Therefore, the recapitulation of the native pancreatic microenvironment and its prompt revascularization should be beneficial for long-term islet survival. In this study, we developed novel bioinks suitable for the microfluidic-assisted multi-material biofabrication of 3D porous pancreatic and vascular structures. The tissue-specific bioactivity was introduced by blending alginate either with pancreatic decellularized extracellular matrix powder (A_ECM)…

3D Printing of Thermoresponsive Polyisocyanide (PIC) Hydrogels as Bioink and Fugitive Material for Tissue Engineering

Polymers 2018 Volume 10, Issue 5, Article 555

Despite the rapid and great developments in the field of 3D hydrogel printing, a major ongoing challenge is represented by the development of new processable materials that can be effectively used for bioink formulation. In this work, we present an approach to 3D deposit, a new class of fully-synthetic, biocompatible PolyIsoCyanide (PIC) hydrogels that exhibit a reverse gelation temperature close to physiological conditions (37 °C). Being fully-synthetic, PIC hydrogels are particularly attractive for tissue engineering, as their properties—such as hydrogel stiffness, polymer solubility, and gelation kinetics—can be precisely tailored according to process requirements. Here, for the first time, we demonstrate…

3D bioprinting of BM-MSCs-loaded ECM biomimetic hydrogels for in vitro neocartilage formation

Biofabrication 2016 Volume 8, Issue 3, 035002

In this work we demonstrate how to print 3D biomimetic hydrogel scaffolds for cartilage tissue engineering with high cell density (>107 cells ml−1), high cell viability (85 ÷ 90%) and high printing resolution (≈100 μm) through a two coaxial-needles system. The scaffolds were composed of modified biopolymers present in the extracellular matrix (ECM) of cartilage, namely gelatin methacrylamide (GelMA), chondroitin sulfate amino ethyl methacrylate (CS-AEMA) and hyaluronic acid methacrylate (HAMA). The polymers were used to prepare three photocurable bioinks with increasing degree of biomimicry: (i) GelMA, (ii) GelMA + CS-AEMA and (iii) GelMA + CS-AEMA + HAMA. Alginate was added…