3D Bioplotter Research Papers

Background: This case report demonstrates the effective clinical application of a 3D-printed, patient-specific polycaprolactone (PCL) resorbable scaffold for staged alveolar bone augmentation. Objective: To evaluate the effectiveness of a 3D-printed PCL scaffold in facilitating alveolar bone regeneration and subsequent dental implant placement. Materials and Methods: A 46-year-old man with a missing tooth (11) underwent staged alveolar bone augmentation using a patient-specific PCL scaffold. Volumetric bone gain and implant stability were assessed. Histological analysis was conducted to evaluate new bone formation and graft integration. Results: The novel approach resulted in a volumetric bone gain of 364.69 ± 2.53 mm3, sufficient to reconstruct the original…

Three Dimensional (3D) bioprinting is one of the most recent additive manufacturing technologies and enables the direct incorporation of cells within a highly porous 3D-bioprinted construct. While the field has mainly focused on developing methods for enhancing printing resolution and shape fidelity, little is understood about the biological impact of bioprinting on cells. To address this shortcoming, this study investigated the in vitro and in vivo response of human osteoblasts subsequent to bioprinting using gelatin methacryloyl (GelMA) as the hydrogel precursor. First, bioprinted and two-dimensional (2D) cultured osteoblasts were compared, demonstrating that the 3D microenvironment from bioprinting enhanced bone-related gene…

Poly(ε-caprolactone) (PCL) chain cleavage results in the formation of polar terminal species, comprising hydroxy and carboxyl groups that enhance surface hydrophilicity and enable subsequent biofunctionalization. However, the direct effects of various acidic and basic treatments on 3D printed PCL scaffolds have not been studied from a functional perspective. In this study, we comprehensively assessed the influence of acid (hydrochloric, HCl) and base (sodium hydroxide, NaOH) catalyzed hydrolysis across different conditions on various properties of 3D printed PCL scaffolds. Analyses included testing of physiochemical and mechanical properties, and assessment of rate and stability of surface-nucleating bioactive apatite-like minerals. HCl exposure resulted…

Objectives This study investigates the design, workflow, and manufacture of highly porous, resorbable additively manufactured, 3‐dimensional (3D) custom scaffolds for the regeneration of large volume alveolar bone defects. Materials and Methods Computed tomography (CT) scans of 5 posterior mandibular vertical bone defects were obtained. Surface masks (3D surface contours) of the recipient site were first isolated using a contrast threshold, transformed into 3D objects, and used to guide the formation of custom implant template models. To determine model accuracy and fit, the gap and overlap between the patient geometry models and the idealized template 3D models were quantified. Models were…