3D Bioplotter Research Papers

Cerebral organoids (cORGs) obtained from induced pluripotent stem cells (iPSCs) have become significant instruments for investigating human neurophysiology, with the possibility of simulating diseases and enhancing drug discovery. The current approaches require a strict process of manual inclusion in animal-derived matrix Matrigel® and are challenged by unpredictability, operators’ skill and expertise, elevated costs, and restricted scalability, impeding their extensive applicability and translational potential. In this study, we present a novel method to generate brain organoids that address these limitations. Our approach does not require a manual, operator-dependent embedding. Instead, it employs a chemically defined hydrogel in which the Matrigel® is…

The fabrication of three-dimensional (3D) bioprinted free-standing, low viscous, cell-laden hydrogels with good resolution, low cytotoxicity, and mechanical properties, comparable to native soft tissues, is a current challenge in tissue engineering. Recently, a new syringe extrusion approach, called Freeform Reversible Embedding of Suspended Hydrogels (FRESH), has been introduced to enhance 3D-bioprinting of soft hydrogels. Printing is conducted with the material embedded in a thermo-reversible gelatin bath, which acts as supporting material and can also initiate in-situ crosslinking when proper crosslinker agents are added. This work is the first to develop a 3D FRESH printable, low-cost, polymeric hydrogel composed of sodium…