3D Bioplotter Research Papers

“There is an immense need for corneal transplants to restore vision after injury or disease. Due to the shortage of donor corneas, three dimensional (3D) bioprinting has emerged as a possible solution to create cornea mimicking structures. The cornea consists of five layers, with the stroma and epithelium forming the two outermost cellular layers. To be able to 3D bioprint these layers, bioinks, and appropriate cell types are needed. For the epithelial layer, human pluripotent stem cell -derived corneal limbal epithelial stem cells (hPSC-LSCs) have great potential. For the stromal layer, human adipose stem cells -derived corneal stromal keratocytes (hASC-CSKs)…

Developing cost-effective and scalable multi-material bioprinting technologies that combine multiple cell types is crucial to produce biomimetic, complex human tissue substitutes and overcome the scarcity of transplantable tissues. These technological developments can revolutionize the treatment of several conditions currently dependent on donor tissues, such as corneal blindness. Here, corneal structures consisting of two layers, stroma and epithelium, were manufactured by extrusion-based 3D bioprinting. To take steps towards clinical translation of bioprinting, three clinically compatible hyaluronic acid based bioinks were combined with human adipose tissue and induced pluripotent stem cell derived cell types. Each of the three bioinks was customized to…