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3D Bioplotter Research Papers
Nanocomposite hydrogel-based bioinks composed of a fucose-rich polysaccharide and nanocellulose fibers for 3D-bioprinting applications
Hydrogels are the most common type of bioinks, yet, finding adequate biomaterials to develop suitable bioinks for 3D bioprinting remains challenging. Herein, innovative hydrogel bioinks were developed by combining nanofibrillated cellulose (NFC) with a fucose-rich polysaccharide, FucoPol (FP), still unexplored for 3D bioprinting. NFC/FP bioinks with different mass proportions, namely 1:1, 2:1, 3:1 and 4:1, were prepared and denominated as NFC1FP, NFC2FP, NFC3FP and NFC4FP. A formulation without NFC was also prepared for comparison purposes (NFC0FP). The rheological properties of the bioinks were enhanced by the addition of NFC, as evidenced by the increase in shear viscosity from 1.39 ± 0.03 Pa s (NFC0FP)…
Biobased hydrogel bioinks of pectin, nanocellulose and lysozyme nanofibrils for the bioprinting of A375 melanoma cell-laden 3D in vitro platforms
Melanoma is one of the most aggressive types of skin cancer, and the need for advanced platforms to study this disease and to develop new treatments is rising. 3D bioprinted tumor models are emerging as advanced tools to tackle these needs, with the design of adequate bioinks being a fundamental step to address this challenging process. Thus, this work explores the synergy between two biobased nanofibers, nanofibrillated cellulose (NFC) and lysozyme amyloid nanofibrils (LNFs), to create pectin nanocomposite hydrogel bioinks for the 3D bioprinting of A375 melanoma cell-laden living constructs. The incorporation of LNFs (5, 10 or 15 wt%) on a…