3D Bioplotter Research Papers

Mechanical strength is a requirement of the bone scaffold for osteoarthritis treatment by the medial open wedge high tibia osteotomy (MOWHTO). The mechanical compression of the scaffold which depends on material concentration and structure must be concerned in MOWHTO substitution due to help to prevent the delayed bone healing process from scaffold collapse. This study was divided into two sections: (i) the influence of varying concentrations between DBM-HA mixed PCL scaffold (20/80, 30/70, and 40/60 % wt./wt. DBM-HA/PCL) on compressive strength, and (ii) the compressive strength of the 0°-90° orientations DBM-HA/PCL scaffold (20/80% wt./wt.) constructed with 300-500 μm pore dimension…

Scale-up of bioengineered grafts toward clinical applications is a challenge in regenerative medicine. Here, we report tissue formation and vascularization of anatomically shaped human tibial condyles ectopically with a dimension of 20 × 15 × 15 mm3. A composite of poly-ɛ-caprolactone and hydroxyapatite was fabricated using layer deposition of three-dimensional interlaid strands with interconnecting microchannels (400 μm) and seeded with human bone marrow stem cells (hMSCs) with or without osteogenic differentiation. An overlaying layer (1 mm deep) of poly(ethylene glycol)-based hydrogel encapsulating hMSCs or hMSC-derived chondrocytes was molded into anatomic shape and anchored into microchannels by gel infusion. After 6 weeks of subcutaneous implantation in athymic rats,…