3D Bioplotter Research Papers

Displaying all papers about Trachea Reconstruction (5 results)

Polymer Materials And Their Usage In Veterinary Practice

Acta Tecnología 2022 Volume: 8, Issue: 4, Pages 109-115

In the field of regenerative medicine and tissue engineering, the use of such materials has been included for a short time, serving not only as a replacement for damaged or missing tissue, but also as a support for the surrounding tissues and cells. Such materials should not only be passively tolerated by the cell, but should also actively promote the growth, differentiation and other processes involved in tissue regeneration. The latest approach is the use and development of bioresorbable and biodegradable polymeric materials. Such materials, with their biocompatibility, degradability and suitable mechanical properties, support the overgrowth of new tissue. The…

In vitro characterization of design and compressive properties of 3D-biofabricated/decellularized hybrid grafts for tracheal tissue engineering

Journal of the Mechanical Behavior of Biomedical Materials 2016 Volume 59, Pages 572–585

Infection or damage to the trachea, a thin walled and cartilage reinforced conduit that connects the pharynx and larynx to the lungs, leads to serious respiratory medical conditions which can often prove fatal. Current clinical strategies for complex tracheal reconstruction are of limited availability and efficacy, but tissue engineering and regenerative medicine approaches may provide viable alternatives. In this study, we have developed a new “hybrid graft” approach that utilizes decellularized tracheal tissue along with a resorbable polymer scaffold, and holds promise for potential clinical applications. First, we evaluated the effect of our decellularization process on the compression properties of…

Tissue‐Engineered Tracheal Reconstruction Using Three‐Dimensionally Printed Artificial Tracheal Graft: Preliminary Report

Artificial Organs 2014 Volume 38, Issue 6, pages E95–E105

Three-dimensional printing has come into the spotlight in the realm of tissue engineering. We intended to evaluate the plausibility of 3D-printed (3DP) scaffold coated with mesenchymal stem cells (MSCs) seeded in fibrin for the repair of partial tracheal defects. MSCs from rabbit bone marrow were expanded and cultured. A half-pipe-shaped 3DP polycaprolactone scaffold was coated with the MSCs seeded in fibrin. The half-pipe tracheal graft was implanted on a 10 × 10-mm artificial tracheal defect in four rabbits. Four and eight weeks after the operation, the reconstructed sites were evaluated bronchoscopically, radiologically, histologically, and functionally. None of the four rabbits showed any…

Intra-scaffold continuous medium flow combines chondrocyte seeding and culture systems for tissue engineered trachea construction

Interactive CardioVasc Thoracic Surgery 2009 Volume 8, Issue 1, Pages 27-30

In this study we tested the possibility of seeding chondrocytes into poly (ethylene glycol)-terephthalate-poly (butylene terephthalate) PEOT/PBT scaffold through an intra-scaffold medium flow and the impact of this continuous medium flow on subsequent chondrocyte-scaffold culture. Eight cubic PEOT/PBT co-polymers (1 cm3) were assigned into two groups. In the semi-dynamic seeding group a continuous medium flow was created inside the scaffolds by a pump system. Around six million chondrocytes were harvested each day, suspended in 1 ml medium and delivered onto the scaffold through the perfusion for a sequential five days. Traditional chondrocytes directly seeding and static culture method was performed…

Anatomical 3D fiber – deposited scaffolds for tissue engineering: designing a neotrachea

Tissue Engineering 2007 Volume: 13 Issue 10, Pages 2483-2493

The advantage of using anatomically shaped scaffolds as compared to modeled designs was investigated and assessed in terms of cartilage formation in an artificial tracheal construct. Scaffolds were rapid prototyped with a technique named three-dimensional fiber deposition (3DF). Anatomical scaffolds were fabricated from a patient-derived computerized tomography dataset, and compared to cylindrical and toroidal tubular scaffolds. Lewis rat tracheal chondrocytes were seeded on 3DF scaffolds and cultured for 21 days. The 3-(4,5-dimethylthiazol-2yl)-2,5-dyphenyltetrazolium bromide (MTT) and sulfated glycosaminoglycan (GAG) assays were performed to measure the relative number of cells and the extracellular matrix (ECM) formed. After 3 weeks of culture, the…