™
3D Bioplotter Research Papers
Displaying all papers by E. S. Gargus (1 results)
Employing PEG crosslinkers to optimize cell viability in gel phase bioinks and tailor post printing mechanical properties
Acta Biomaterialia
2019
Volume 99, Pages 121-132
The field of 3D bioprinting has rapidly grown, yet the fundamental ability to manipulate material properties has been challenging with current bioink methods. Here, we change bioink properties using our PEG cross-linking (PEGX) bioink method with the objective of optimizing cell viability while retaining control of mechanical properties of the final bioprinted construct. First, we investigate cytocompatible, covalent cross-linking chemistries for bioink synthesis (e.g. Thiol Michael type addition and bioorthogonal inverse electron demand Diels-Alder reaction). We demonstrate these reactions are compatible with the bioink method, which results in high cell viability. The PEGX method is then exploited to optimize extruded…