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3D Bioplotter Research Papers
Displaying all papers by J. Kuestermeyer (1 results)
Calvaria bone chamber-A new model for intravital assessment of osseous angiogenesis
Journal of Biomedical Materials Research Part A
2011
Volume 99A, Issue 2, pages 151-157
The faith of tissue engineered bone replacing constructs depends on their early supply with oxygen and nutrients, and thus on a rapid vascularization. Although some models for direct observation of angiogenesis are described, none of them allows the observation of new vessel formation in desmal bone. Therefore, we developed a new chamber model suitable for quantitative in vivo assessment of the vascularization of bone substitutes by intravital fluorescence microscopy. In the parietal calvaria of 32 balb/c mice a critical size defect was set. Porous 3D-poly(L-lactide-co-glycolide) (PLGA)-blocks were inserted into 16 osseous defects (groups 3 and 4) while other 16 osseous…