3D Bioplotter Research Papers

The realization of a fully bioengineered, functional corneal tissue is challenging. The development of full-thickness corneal models requires appropriate material fabrication methods as well as cell culture protocols. Here we describe the construction of a full-thickness corneal model (FCM) using electro-compacted (EC) collagen films. EC collagen films support primary human corneal epithelial cell (hCEpiC) stratification, guide primary human corneal stromal cell (hCSC) orientation and maintain primary human corneal endothelial cell (hCEndC) growth and confluence. Using fibrinogen as a tissue adhesive we assembled all three layers to produce a full-thickness corneal construct with structural integrity. Importantly, the FCM is highly transparent…

Synthesis of bioinks for bioprinting of respiratory tissue requires considerations related to immunogenicity, mechanical properties, printability, and cellular compatibility. Biomaterials can be tailored to provide the appropriate combination of these properties through the synergy of materials with individual pros and cons. Sodium alginate, a water-soluble polymer derived from seaweed, is a cheap yet printable biomaterial with good structural properties; however, it lacks physiological relevance and cell binding sites. Collagen, a common component in the extra cellular matrix of many tissues, is expensive and lacks printability; however, it is highly biocompatible and exhibits sites for cellular binding. This paper presents our…

With the increasing applications of 3D printing technology in biomedical field, the composition or additives of the related materials has become critical for the next development. In the current study, we have prepared 3D printed polycaprolactone-hydroxyapatite (PCL-HA) porous scaffolds with loaded heparan sulfate (HS), in order to reveal the reparative effect of different concentrations of HS on the healing of bone defects. As a result, the scaffold itself showed sound compression resistance, air porosity and good biocompatibility. From both in vitro and in vivo experiments, the scaffold with low concentration of HS led to positive effects in promoting osteoblast maturation…