3D Bioplotter Research Papers

Organ-on-a-chip stands as a pivotal platform for skeletal muscle research while constructing 3D skeletal muscle tissues that possess both macroscopic and microscopic structures remains a considerable challenge. This study draws inspiration from LEGO-like assembly, employing a modular approach to construct muscle tissue that integrates biomimetic macroscopic and microscopic structures. Modular LEGO-like hybrid nanofibrous scaffold bricks were fabricated by the combination of 3D printing and electrospinning techniques. Skeletal muscle cells cultured on these modular scaffold bricks exhibited a highly orientated nanofibrous structure. A variety of construction of skeletal muscle tissues further enabled development by various assembling processes. Moreover, skeletal muscle-on-a-chip (SMoC)…

Cervical atresia is a rare congenital Müllerian duct anomaly that manifests as the absence or deformed nonfunctional presence of the cervix. Herein, a multi-layered biodegradable stent is fabricated using a homogeneous blend of silk fibroin with polycaprolactone using hexafluoroisopropanol as a common solution. Briefly, a concentric cylinder of 3D honeycomb layer is sandwiched within electrospun sheets for fixing at the cervico-uterine junction to pave the way of cervical reconstruction. An average length of 40 mm with 3 mm diameter is fabricated for the hybrid stent design. SEM evidences an evenly distributed pore architecture of the electrospun layer, and mechanical characterization…

In this study, two-component, morphologically composite scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with a nitrogen-argon (N2-Ar) plasma to enhance their surface properties. The 3D-printed component provided mechanical strength, while the electrospun fibrous component acted as a mimic to the extracellular matrix to improve cell-substrate interactions. Two biodegradable polyesters, poly(L-lactide-co–ε-caprolactone) (PLCL) and poly(L-lactide-co-glycolide) (PLGA), were used to create the scaffolds. The resulting 3D/E/N2-Ar scaffolds were characterized in terms of surface properties (morphology, chemical compositions, wettability, roughness, crystallinity), degradation, mechanical properties, and cell cytotoxicity, cell attachment and proliferation, LDH release and cell apoptosis.…

Fibre alignment technology is crucial in various emerging applications, such as drug delivery systems, tissue engineering, and scaffold fabrication. However, conventional methods have limitations when it comes to incorporating aligned fibres into 3D printed structures in situ. This research demonstrates the use of custom-designed templates made with conductive ink to control the alignment of drug-loaded polymer fibres on a 3D printed microscale structure. Three different geometries were designed, and the effects of the template on fibre diameter and pattern were investigated. The hybrid structure demonstrated successful control of aligned fibres on printed structures using grounded conductive ink geometric electrodes, as…