3D Bioplotter Research Papers

Low-intensity pulsed ultrasound (LIPUS) assisted bone repair is confirmed effective in clinic. Here, a 3D-printed composite poly(DL-lactic acid)/mesoporous bioactive glass scaffold was constructed for particular use in LIPUS-assisted bone tissue engineering. The scaffolds contain dimethyloxallyl glycine (DMOG) loaded microbubbles in pores, which can be released after implanting via LIPUS stimulation. Local DMOG concentrations are modulated through ultrasound power and processing time. The rat bone marrow-derived mesenchymal stem cells (rBMSCs) on these scaffolds with ultrasound treatment show improved proliferation and early osteogenic differentiation.

Self-setting calcium phosphate cement (CPC) scaffold with interconnected macropores is hard to prepare without compromising its hydration reaction. Herein, an indirect 3D printing method is using to prepare CPC scaffolds. Detailedly, polycaprolactone (PCL)-sacrificed models with different strut sizes are first printed by 3D plotting technique, and then the CPC pastes are perfused into the PCL models and then self-setting. After the removal of the PCL models, the CPC scaffolds with different pore sizes are obtained. It is showed in the results that the prepared CPC scaffolds had uniform shape and 3D interconnected macropore structure. Meanwhile, the compressive strength of CPC…

Background To investigate the effect of low-intensity pulsed ultrasound (LIPUS) combined with lipid microbubbles on the proliferation and bone regeneration of bone marrow mesenchymal stem cells (BMSCs) in poly (lactic-glycolic acid copolymer) (PLGA)/α-tricalcium phosphate (TCP) 3D-printed scaffolds. Methods BMSCs were irradiated with different LIPUS parameters and microbubble concentrations, and the best acoustic excitation parameters were selected. The expression of type I collagen and the activity of alkaline phosphatase were detected. Alizarin red staining was used to evaluate the calcium salt production during osteogenic differentiation. Results BMSCs proliferation was the most significant under the condition of 0.5% (v/v) lipid microbubble concentration,…