3D Bioplotter Research Papers

Osteocytes, essential regulators of bone homeostasis, are embedded in the mineralized bone matrix. Given the spatial arrangement of osteocytes, bioprinting represents an ideal method to biofabricate a 3D osteocyte network with a suitable surrounding matrix similar to native bone tissue. Here, we reported a 3D bioprinted osteocyte-laden hydrogel for biomimetic mineralization in vitro with exceptional shape fidelity, a high cell density (107 cells per ml) and high cell viability (85–90%). The bioinks were composed of biomimetic modified biopolymers, namely, gelatine methacrylamide (GelMA) and hyaluronic acid methacrylate (HAMA), with or without type I collagen. The osteocyte-laden constructs were printed and cultured…

Highlights * An elastic degradable polyurethane (PU) bearing pendent HSNGLPL peptide for TGF-β1 affinity binding mimics the extracellular matrix function to retain and release growth factors. * The pendant peptide sequence presented a high affinity for TGF-β1 retaining, even when the surface was pre-coated with other proteins. * The synthesized PU shows good extrusion processing ability and can be printed into 3D scaffolds with designed porous structures. * The released TGF-β1 from surface conjugating was tested by differentiation guiding experiments of ATDC5 cells in vitro and the regeneration of the surrounding tissue after implanting in vivo.