™
3D Bioplotter Research Papers
Coffee parchment-derived nanocellulose as reinforcing agent in hydrogel inks for extrusion-based 3D printing of biphasic osteochondral scaffolds
Nano-reinforcement plays a crucial role in enhancing the mechanical properties, printability, and structural integrity of hydrogels for scaffold fabrication. This study explores the potential of TEMPO-oxidized nanocellulose (TONCs) derived from coffee parchment as a reinforcing agent in sodium alginate hydrogels for extrusion-based 3D printing of biphasic osteochondral scaffolds. TONCs were synthesized via TEMPO-mediated oxidation using sodium hypochlorite at 5, 10, 15, and 20 mmol/g, yielding cellulose nanofibers (TOCNFs: TONC-5, TONC-10) and cellulose nanocrystals (TOCNCs: TONC-15, TONC-20). Rheological analysis revealed that TONC-10-reinforced hydrogels exhibited the highest yield stress (75.2 Pa), consistency coefficient (323.8 Pa sⁿ), and printability index (0.929), attributed to…
Efficient dual crosslinking of protein–in–polysaccharide bioink for biofabrication of cardiac tissue constructs
Myocardial infarction (MI) is a lethal cardiac disease that causes most of the mortality across the world. MI is a consequence of plaque in the arterial walls of heart, which eventually result in occlusion and ischemia to the myocardial tissues due to inadequate nutrient and oxygen supply. As an efficient alternative to the existing treatment strategies for MI, 3D bioprinting has evolved as an advanced tissue fabrication technique where the cell–laden bioinks are printed layer–by–layer to create functional cardiac patches. In this study, a dual crosslinking strategy has been utilized towards 3D bioprinting of myocardial constructs by using a combination…
Alginate and Nanocellulose Dressings With Extract From Salmon Roe Reduce Inflammation and Accelerate Healing of Porcine Burn Wounds
Partial-thickness thermal burn wounds are characterized by a prolonged inflammatory response, oxidative stress, tissue damage, and secondary necrosis. An optimal dressing for burn wounds would reduce inflammation and oxidative stress while providing a moist, absorbent, and protective cover. We have developed an extract from unfertilized salmon roe containing components with potential anti-inflammatory and antioxidative properties, called HTX. HTX has been combined with alginate from brown algae and nanocellulose from tunicates, and 3D printed into a solid hydrogel wound dressing called Collex. Here, Collex was tested on partial thickness burn wounds in Göttingen minipigs compared to Jelonet, and a variant of…