3D Bioplotter Research Papers

Despite recent advances in extrusion bioprinting of cell-laden hydrogels, using naturally derived bioinks to biofabricate complex elastic tissues with both satisfying biological functionalities and superior mechanical properties is hitherto an unmet challenge. Here, we address this challenge with precisely designed biological tough hydrogel bioinks featuring a double-network structure. The tough hydrogels consisted of energy-dissipative dynamically crosslinked glycosaminoglycan hyaluronic acid (o-nitrobenzyl-grafted hyaluronic acid) and elastin through Schiff’s base reaction, and free-radically polymerized gelatin methacryloyl. The incorporation of elastin further improved the elasticity, stretchability (∼170% strain), and toughness (∼45 kJ m−3) of the hydrogels due to the random coiling structure. We used this novel…

Complex shaped bone defects that need to be filled are very common in clinic. But after filling, gaps are inevitably left between substitutes and host bone due to the poor conformability of preformed implants, hence hindering bone regeneration. Therefore, based on our previous study, we here used the bioink (named PPG) composed of polyurethane, polyacrylamide, and gelatin with optimized composition ratio to three-dimensionally (3D) print an inorganic/organic composite hydrogel scaffold with self-expandability to fill irregular bone defects and bioactivity to accelerate bone healing through adjusting the content of bioactive ceramic (BC). The results indicated that, the 3D printed BC/PPG scaffold…

Three-dimensional (3D) functional graphene-based architecture with superior electrical conductivity and good mechanical strength has promising applications in energy storage and electrics. Viscoelasticity-adjustable inks make it possible to achieve desired 3D architectures with interconnected and continuous interior networks by micro-extrusion printing. In this work, ultra-low-concentration graphene oxide (GO) inks of ~ 15 mg·ml−1 have been obtained and demonstrated in direct 3D printing with a facile cross-linking (direct ink writing). The rheological behavior of the GO strategy by cations, which is the lowest concentration to achieve direct ink writing inks, could be adjusted from 1×104 to 1×105 Pa·s−1 with different concentrations of cations due to strong…

Tissue-engineered ear cartilage scaffold based on three-dimensional (3D) bioprinting technology presents a new strategy for ear reconstruction in individuals with microtia. Natural hydrogel is a promising material due to its excellent biocompatibility and low immunogenicity. However, insufficient mechanical property required for cartilage is one of the major issues pending to be solved. In this study, the gelatin methacryloyl (GelMA) hydrogel reinforced with bacterial nanocellulose (BNC) was developed to enhance the biomechanical properties and printability of the hydrogel. The results revealed that the addition of 0.375% BNC significantly increased the mechanical properties of the hydrogel and promoted cell migration in the…

Tissue engineering provides a promising strategy for auricular reconstruction. Although the first international clinical breakthrough of tissue-engineered auricular reconstruction has been realized based on polymer scaffolds, this approach has not been recognized as a clinically available treatment because of its unsatisfactory clinical efficacy. This is mainly since reconstruction constructs easily cause inflammation and deformation. In this study, we present a novel strategy for the development of biological auricle equivalents with precise shapes, low immunogenicity, and excellent mechanics using auricular chondrocytes and a bioactive bioink based on biomimetic microporous methacrylate-modified acellular cartilage matrix (ACMMA) with the assistance of gelatin methacrylate (GelMA),…

Malignant bone tumors have caused great obstacles and serious illnesses for tumor recurrence and difficulty in reconstructing and repairing large defects after tumorectomy. Additionally, long-term efficacy, satisfactory biocompatibility and excellent properties for anti-tumor agents are necessary in the biomedical field. To solve these problems, a novel idea has been proposed on building an integrative anti-tumor/bone repairing scaffold by covering photothermal therapy (PTT) composite MoS2-PLGA film on the surface of borosilicate bioactive glass (BG). In our study, the MoS2-integrated composite BG (BGM) scaffolds can rapidly and effectively elevate temperature, and they exhibited excellent photothermal stability, under 808 nm laser irradiation. Notably,…

The high surface area ratio and special structure of mesoporous bioactive glass (MBG) endow it with excellent physical adsorption of various drugs without destroying the chemical activity. Silicate 1393 bioactive glass (1393) is famous for its fantastic biodegradability and osteogenesis. Herein, we have built a novel vehicle-like drug delivery 3D printing scaffold with multiplexed drug delivery capacity by coating MBG on the surface of 1393 (1393@MBG). Furthermore, we have applied DEX and BMP-2 on the 1393@MBG scaffold to endow it with antibacterial and osteogenic properties. Results indicated that this 1393@MBG scaffold could effectively load and controlled release BMP-2, DNA and…