3D Bioplotter Research Papers

Breath analysis is a non-invasive tool used in medical diagnosis. However, the current generation of breath analyzers is expensive, time-consuming, and requires sample gas separation. In this work, a simple, yet effective, low-cost ammonia gas sensor based on poly(2-acrylamido-2-methyl-1-propanesulfonic acid) is presented for non-invasive medical diagnosis. The designed sensor has a broad detection range to ammonia gas up to 1000 ppm with a limit of detection of 30 ppb. This is a robust sensor, which functions at high relative humidity (RH) (>90%) and exhibits consistent electrical responses under different test conditions. The result of a blind test validates the sensor’s…

Self-setting calcium phosphate cement (CPC) scaffold with interconnected macropores is hard to prepare without compromising its hydration reaction. Herein, an indirect 3D printing method is using to prepare CPC scaffolds. Detailedly, polycaprolactone (PCL)-sacrificed models with different strut sizes are first printed by 3D plotting technique, and then the CPC pastes are perfused into the PCL models and then self-setting. After the removal of the PCL models, the CPC scaffolds with different pore sizes are obtained. It is showed in the results that the prepared CPC scaffolds had uniform shape and 3D interconnected macropore structure. Meanwhile, the compressive strength of CPC…

Distributed sensing of environmental parameters is going towards solutions that are more efficient by taking inspiration from flying plant seeds. Yet, present technologies mostly rely on electronics, and they are often heavy and not biodegradable. Here, we develop a biodegradable and porous material, based on cellulose acetate and lignin, and characterize its degree of porosity. We use this material to 3D print lightweight and porous artificial fliers inspired by Ailanthus altissima seeds. By 3D printing, we can tailor in a precise way the morphology of the artificial flier that strongly influences its aerodynamic behavior. We add a cellulose-based photonic crystal…

Nerve conduits may represent a valuable alternative to autograft for the regeneration of long-gap damages. However, no NCs have currently reached market approval for the regeneration of limiting gap lesions, which still represents the very bottleneck of this technology. In recent years, a strong effort has been made to envision an engineered graft to tackle this issue. In our recent work, we presented a novel design of porous/3D-printed chitosan/poly-ε-caprolactone conduits, coupling freeze drying and additive manufacturing technologies to yield conduits with good structural properties. In this work, we studied genipin crosslinking as strategy to improve the physiochemical properties of our…

Bulk Ni samples were additively manufactured using particle-based ink extrusion. Three samples were characterized for thermoelectric transport properties including electrical resistivity, thermal conductivity, Seebeck coefficient, and thermoelectric figure of merit. Sample-to-sample deviations in transport were small but observable; these were attributed to stochastic porosity from the manufacturing method. Transport results were compared to previously published results in both porous and dense Ni, indicating that the salient features in the traditionally manufactured Ni samples are maintained in their additively manufactured counterparts. These results are offered as evidence of the feasibility of using particle-based ink extrusion additive manufacturing for thermoelectric applications.

The repair of nasal cartilage lesions and defects is still a difficult problem in nasal surgery, and nasal cartilage tissue engineering will be an effective way to solve this problem. Hydrogel has excellent application potential in tissue engineering. In order to produce a 3D printable scaffold for cartilage regeneration, we prepared gelatin methacryloyl (GelMA)/hyaluronic acid methacryloyl (HAMA)/bacterial cellulose (BC) composite hydrogel. The composite hydrogel was characterized by swelling, mechanical properties, and printing performance test. Compared with GelMA/HAMA hydrogel, the addition of BC not only significantly enhanced the mechanical properties of the hydrogels, but also improved the printing fidelity. At the…

Decellularized extracellular matrices (ECMs) are being extensively used for tissue engineering purposes and detergents are predominantly used for this. A facile detergent-free decellularization method is developed for dermal matrix and compared it with the most used detergent-based decellularization methods. An optimized, single-step, cost-effective Hypotonic/Hypertonic (H/H) Sodium Chloride (NaCl) solutions-based method is employed to decellularize goat skin that resulted in much higher yield than other methods. The ECM composition, mechanical property, and cytocompatibility are evaluated and compared with other decellularization methods. Furthermore, this H/H-treated decellularized dermal ECM (ddECM) exhibits a residual DNA content of <50 ng mg−1 of dry tissue. Moreover, 85.64 ± 3.01% of glycosaminoglycans…

Large size bone defects affect human health and remain a worldwide health problem that needs to be solved immediately. 3D printing technology has attracted substantial attention for preparing penetrable multifunctional scaffolds to promote bone reconditioning and regeneration. Inspired by the spongy structure of natural bone, novel porous degradable scaffolds have been printed using polymerization of lactide and caprolactone (PLCL) and bioactive glass 45S5 (BG), and polydopamine (PDA) was used to decorate the PLCL/BG scaffolds. The physicochemical properties of the PLCL/BG and PLCL/BG/PDA scaffolds were measured, and their osteogenic and angiogenic effects were characterized through a series of experiments both in…

3D bioprinting enables the fabrication of biomimetic cell-laden constructs for cartilage regeneration, offering exclusive strategies for precise pharmacological screenings in osteoarthritis (OA). Synovial inflammation plays a crucial role in OA’s early stage and progression, characterized by the increased of the synovial pro-inflammatory mediators and cytokines and chondrocyte apoptosis. Therefore, there is an urgent need to develop solutions for effectively managing the primary events associated with OA. To address these issues, a phenolic-based biocompatible ionic liquid approach, combining alginate (ALG), acemannan (ACE), and cholinium caffeate (Ch[Caffeate]), was used to produce easily printable bioinks. Through the use of this strategy 3D constructs…

Objective. The objective of this study was to compare the host inflammatory response of our previously described hyperelastic, 3D-printed (3DP) hydroxyapatite (HA)-demineralized bone matrix (DBM) composite scaffold to the response elicited with the use of recombinant human bone morphogenetic protein-2 (rhBMP-2) in a preclinical rat posterolateral lumbar fusion model. Summary of Background Data. Our group previously found that this 3D-printed HA-DBM composite material shows promise as a bone graft substitute in a preclinical rodent model, but its safety profile had yet to be assessed. Methods. Sixty female Sprague-Dawley rats underwent bilateral posterolateral intertransverse lumbar spinal fusion using with the following…